关键词: 肝癌动物模型, 不饱和脂肪酸, 肝癌移植瘤, 重症联合免疫缺陷小鼠 

Abstract: Objective To establish SCID mice tumor model for screening new antitumor drugs. Method Liver cancer tissue was transplanted subcutaneously into severe combined immune deficiency ( SCID) mice. When the transplanted tumor grew to 6-7 mm, fifty SCID tumor-bearing mice were divided into unsaturated fatty acid low, medium and high dose groups [50 mg / ( kg·d) , 100 / ( kg·d) , 200 mg / ( kg·d) ] . Pentafluorouracil ( 5-FU) group [100 mg / ( kg·d) ] and model group [ distilled water 2 mL / ( kg·d) ] were administered continuously for 21 d. The transplanted tumor was removed to measure its quality and volume, and the tumor growth was compared. The protein expression levels of P21, P53, P13K and PKB / Akt in tumor tissues were determined by enzyme-linked immunosorbent assay (ELISA) . Result The weight or volume of liver cancer transplanted tumor in low, medium and high dose of unsaturated fatty acids groups and 5 -FU group was significantly lower than that in model group (P<0. 01) . The level of P21 was significantly higher compared with model group (P<0. 05, P<0. 01) ,the levels of P53, P13K and PKB / Akt were significantly lower than those in model group (P<0. 05, P<0. 01) . Conclusion Unsaturated fatty acids can inhibit the growth of liver cancer transplanted tumor, The possible mechanism is related to up-regulation of P21 level, down-regulation of P53, P13K, PKB / Akt levels, and inhibition of PI3K-Akt signal transduction pathway activity.

Key words: liver cancer animal model, unsaturated fatty acids, liver cancer transplanted tumor, severe combined immune deficiency mice